產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Adrenocorticotropic Hormone (ACTH)
促腎上腺皮質(zhì)激素(ACTH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號：
CEA836Ra-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for Adrenocorticotropic Hormone (ACTH) |
| 基因別稱： | Corticotropin; Adrenocorticotropin |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 檢測范圍： | 12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 5.25pg/mL |
| 適應生物： | Rattus norvegicus (Rat ，大鼠) |
| 樣本： | Serum, plasma and other biological fluids |
| 實驗時長： | 2hours |
| 種類： | 參考價格：2553.6/48T ; 3648/96T ; 16416/96T*5 ; 31008/96T*10 ; 255360/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone Releasing Hormone (GHRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone Releasing Hormone (GHRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 4度和-20度分開保存，避免反復凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標本量： | 詳見說明書 |
| 需自備的設備及試劑： | 1、450±10nm濾光片的酶標儀(建議儀器使用前提前預熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Endocrinology;Hormone metabolism;Urology; |
| Emit： | Adrenocorticotropic Hormone |
