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| 英文名稱： | Mini Samples ELISA Kit for Transcription Factor P65 (NFKB3) |
| 基因別稱： | Nuclear factor NF-kappa-B p65 subunit; NFKBP65; RELA; p65; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 |
| 實(shí)驗(yàn)步驟： | 1. Prepare all reagents, samples and standards; 2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C; 3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 20µL Stop Solution. Read at 450nm immediately. |
| 檢測(cè)范圍： | 0.156-10ng/mL |
| 最低檢測(cè)限： | The minimum detectable dose of this kit is typically less than 0.058ng/mL |
| 適應(yīng)生物： | Homo sapiens (Human) |
| 樣本： | tissue homogenates, cell lysates and other biological fluids |
| 種類： | Double-antibody Sandwich |
| 特異性： |
This assay has high sensitivity and excellent specificity for detection of Mini Samples Transcription Factor P65 (NFKB3). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Transcription Factor P65 (NFKB3) were tested 20 times on one plate, respectively. |
| 穩(wěn)定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 實(shí)驗(yàn)原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Transcription Factor P65 (NFKB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Transcription Factor P65 (NFKB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Transcription Factor P65 (NFKB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Transcription Factor P65 (NFKB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 產(chǎn)品應(yīng)用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 應(yīng)用備注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期貨： | 5-7天 |
| 運(yùn)輸條件： | 藍(lán)冰運(yùn)輸 |
| 保存條件： | 按要求4度和-20度分開(kāi)保存 |
| 有效期： | 12個(gè)月 |
| 限制： | 僅用科研 ( ONLY USE R&D research ) |
| 需自備的設(shè)備及試劑： | 450nm酶標(biāo)儀 & 移液器等 |
| Research_Areas： | Apoptosis; |
| Emit： | Transcription Factor P65 |
| Excite： | Q04206 |
