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| 英文名稱： | ELISA Kit for Phosphatidylinositol Trisphosphate (PIP3) |
| 基因別稱： | PtdIns(3,4,5)P3 |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 檢測范圍： | 6.17-500ng/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 2.09ng/mL |
| 適應(yīng)生物： | Pan-species (General) |
| 樣本： | serum, plasma and other biological fluids |
| 實驗時長： | 2h |
| 種類： | Competitive Inhibition |
| 特異性： |
This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol Trisphosphate (PIP3). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol Trisphosphate (PIP3) were tested 20 times on one plate, respectively. |
| 穩(wěn)定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 實驗原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Phosphatidylinositol Trisphosphate (PIP3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Phosphatidylinositol Trisphosphate (PIP3) and unlabeled Phosphatidylinositol Trisphosphate (PIP3) (Standards or samples) with the pre-coated antibody specific to Phosphatidylinositol Trisphosphate (PIP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample. |
| 產(chǎn)品應(yīng)用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 應(yīng)用備注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期貨： | 5-7天 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 按要求4度和-20度分開保存 |
| 有效期： | 12個月 |
| 限制： | 僅用科研 ( ONLY USE R&D research ) |
| Comment： | 需要代測請聯(lián)系說明 |
| 需自備的設(shè)備及試劑： | 450nm酶標儀 & 移液器等 |
| Emit： | Phosphatidylinositol Trisphosphate |
| Excite： | - |
