產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b)
氫/鉀離子交換ATP酶β肽(ATP4b)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號：
SEG384Mu-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) |
| 基因別稱： | Proton pump beta chain; Gastric H(+)/K(+) ATPase subunit beta |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
| 檢測范圍： | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than0.066ng/mL |
| 適應(yīng)生物： | Mus musculus (Mouse ，小鼠) |
| 樣本： | Tissue homogenates and other biological fluids. |
| 實驗時長： | 3hours |
| 種類： | 參考價格：3024/48T ; 4320/96T ; 19440/96T*5 ; 36720/96T*10 ; 302400/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b). No significant cross-reactivity or interference between ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroporphyrinogen Decarboxylase (UROD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroporphyrinogen Decarboxylase (UROD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroporphyrinogen Decarboxylase (UROD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uroporphyrinogen Decarboxylase (UROD) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應(yīng)用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 4度和-20度分開保存，避免反復(fù)凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標本量： | 詳見說明書 |
| 需自備的設(shè)備及試劑： | 1、450±10nm濾光片的酶標儀(建議儀器使用前提前預(yù)熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Signal transduction; |
