產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Caspase 14 (CASP14)
胱天蛋白酶14(CASP14)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
貨 號：
SCA457Hu-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | CLIA Kit for Caspase 14 (CASP14) |
| 基因別稱： | Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14 |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC; 8. Read RLU value immediately. |
| 檢測范圍： | 312.50-20000pg/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 10.56pg/mL |
| 適應(yīng)生物： | Homo sapiens (Human , 人) |
| 樣本： | Tissue homogenates, cell lysates and other biological fluids |
| 實驗時長： | 2h, 40min |
| 種類： | 參考價格：3528/48T ; 5040/96T ; 22680/96T*5 ; 42840/96T*10 ; 352800/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of Caspase 14 (CASP14). No significant cross-reactivity or interference between Caspase 14 (CASP14) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) level in the sample or standard. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應(yīng)用： | CLIA ，化學(xué)發(fā)光 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運(yùn)輸條件： | 藍(lán)冰運(yùn)輸 |
| 保存條件： | 4度和-20度分開保存，避免反復(fù)凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標(biāo)本量： | 詳見說明書 |
| 需自備的設(shè)備及試劑： | 1、微孔板型多功能化學(xué)發(fā)光儀(參數(shù)：滯后時間 30.0secs；讀數(shù)時間1.0 sec/well); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Apoptosis; |
| Emit： | Caspase 14 |
