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| 英文名稱： | Mini Samples ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) |
| 基因別稱： | DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2 |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C; 3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 20µL Stop Solution. Read at 450nm immediately. |
| 檢測范圍： | 15.6-1,000pg/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 5.8pg/mL |
| 適應(yīng)生物： | Homo sapiens (Human) |
| 樣本： | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 實驗時長： | 3h |
| 種類： | Double-antibody Sandwich |
| 特異性： |
This assay has high sensitivity and excellent specificity for detection of Mini Samples Tumor Necrosis Factor Alpha (TNFa). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively. |
| 穩(wěn)定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 實驗原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 產(chǎn)品應(yīng)用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 應(yīng)用備注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期貨： | 5-7天 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 按要求4度和-20度分開保存 |
| 有效期： | 12個月 |
| 限制： | 僅用科研 ( ONLY USE R&D research ) |
| 需自備的設(shè)備及試劑： | 450nm酶標儀 & 移液器等 |
| Research_Areas： | Cytokine;Tumor immunity;Infection immunity; |
| Emit： | Tumor Necrosis Factor Alpha |
| Excite： | P01375 |
