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| 英文名稱： | Instant ELISA Kit for High Mobility Group Protein 1 (HMGB1) |
| 基因別稱： | HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
| 檢測范圍： | 62.5-4,000pg/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 22.5pg/mL |
| 適應(yīng)生物： | Homo sapiens (Human) |
| 樣本： | serum, plasma and other biological fluids |
| 實驗時長： | 1h, 10min |
| 種類： | Double-antibody Sandwich |
| 特異性： |
This assay has high sensitivity and excellent specificity for detection of Instant High Mobility Group Protein 1 (HMGB1). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant High Mobility Group Protein 1 (HMGB1) were tested 20 times on one plate, respectively. |
| 穩(wěn)定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 實驗原理： | The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant High Mobility Group Protein 1 (HMGB1). Standards or samples and HRP-labeled detection antibody specific to Instant High Mobility Group Protein 1 (HMGB1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant High Mobility Group Protein 1 (HMGB1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant High Mobility Group Protein 1 (HMGB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 產(chǎn)品應(yīng)用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 應(yīng)用備注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期貨： | 5-7天 |
| 運輸條件： | 藍(lán)冰運輸 |
| 保存條件： | 按要求4度和-20度分開保存 |
| 有效期： | 12個月 |
| 限制： | 僅用科研 ( ONLY USE R&D research ) |
| 需自備的設(shè)備及試劑： | 450nm酶標(biāo)儀 & 移液器等 |
| Research_Areas： | Infection immunity; |
| Emit： | High Mobility Group Protein 1 |
| Excite： | P09429 |
