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產品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > USCN & ELISA試劑盒 > 酶聯免疫試劑盒/elisa試劑盒(夾心法）

酪氨酸3/色氨酸5單加氧酶激活蛋白ζ(YWHAz)檢測試劑盒(酶聯免疫吸附試驗法)

貨號：

USCN-SEJ809Mi

規(guī) 格：

96wells

品牌：

Uscn

產品描述
參考文獻
相關產品
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說明書下載

英文名稱：

ELISA Kit for Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

基因別稱：

KCIP-1; 14-3-3-zeta; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1

實驗步驟：

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

檢測范圍：

31.2-2,000pg/mL

最低檢測限：

The minimum detectable dose of this kit is typically less than 11.3pg/mL

適應生物：

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

樣本：

tissue homogenates, cell lysates and other biological fluids

實驗時長：

種類：

Double-antibody Sandwich

特異性：

This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz).
No significant cross-reactivity or interference between Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

穩(wěn)定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

實驗原理：

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

產品應用：

Enzyme-linked immunosorbent assay for Antigen Detection.

應用備注：

48T, 96T, 96T×5, 96T×10, 96T×100

期貨：

5-7天

運輸條件：

藍冰運輸

保存條件：

按要求4度和-20度分開保存

有效期：

12個月

限制：

僅用科研 ( ONLY USE R&D research )

需自備的設備及試劑：

450nm酶標儀 & 移液器等

Research_Areas：

Enzyme & Kinase;

Emit：

Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta

Excite：

P63104

一、ELISA試劑盒代測（凡購買本公司的產品）

1. 提供代測產品的貨號，英文全名及縮寫；

2. 提供要檢測的樣本信息（比如：樣本類型，數量，保存條件，保存時間等）；

3. 提供聯系人的全名，單位，電話等信息；

4. 簽訂代測協議；

5. 樣本需干冰運輸，收到標本后一周內安排檢測實驗，十個工作日左右出具檢測報告。

二、免疫組化實驗（免費）

購買本公司的產品。

三、售前、售中、售后的技術支持（隨時）

說明書下載

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內毒素檢測專用耗材

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