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| 英文名稱： | ELISA Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) |
| 基因別稱： | BCKDHKIN; 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
| 檢測范圍： | 3.12-200ng/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 1.29ng/mL |
| 適應(yīng)生物： | Rattus norvegicus (Rat) |
| 樣本： | Tissue homogenates, cell lysates and other biological fluids. |
| 實驗時長： | 3h |
| 種類： | Double-antibody Sandwich |
| 特異性： |
This assay has high sensitivity and excellent specificity for detection of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) were tested 20 times on one plate, respectively. |
| 穩(wěn)定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 實驗原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 產(chǎn)品應(yīng)用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 應(yīng)用備注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期貨： | 5-7天 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 按要求4度和-20度分開保存 |
| 有效期： | 12個月 |
| 限制： | 僅用科研 ( ONLY USE R&D research ) |
| 需自備的設(shè)備及試劑： | 450nm酶標儀 & 移液器等 |
| Research_Areas： | Enzyme & Kinase; |
| Emit： | Branched Chain Alpha-Ketoacid Dehydrogenase Kinase |
| Excite： | Q00972 |
