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大腸桿菌殘留蛋白(ECP)檢測試劑盒
貨 號：
SEX655Ge-96T*5
規(guī) 格：
96T*5
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for Escherichia coli Protein (ECP) |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50μL Stop Solution. Read at 450nm immediately. |
| 檢測范圍： | 0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL. |
| 適應生物： | Pan-species (General，通用) |
| 樣本： | biological agents |
| 種類： | 夾心法 |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of ECP. No significant cross-reactivity or interference between ECP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between ECP and all the analogues, therefore, cross reaction may still exist. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ECP were tested 20
times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ECP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within
the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | The microtiter plate provided in this kit has been pre-coated with an antibody specific to ECP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ECP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ECP in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 產(chǎn)品應用： | elisa |
| 應用備注： | 1. We are only responsible for the kit itself, but not for the samples consumed during the assay. The user should
calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in
advance. 2. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. Sample should be diluted by 0.01mol/L PBS(PH=7.0-7.2). 3. If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary. 4. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals. 5. Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products. 6. Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit. 7. Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results. |
| 期貨： | 5-7個工作日 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 4度 |
| 有效期： | 6個月 |
| 限制： | 僅用于科研 |
| 需自備的設備及試劑： | 1. Microplate reader with 450 ± 10nm filter. 2. Precision single or multi-channel pipettes and disposable tips. 3. Eppendorf Tubes for diluting samples. 4. Deionized or distilled water. 5. Absorbent paper for blotting the microtiter plate. 6. Container for Wash Solution |
