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牛血清白蛋白(BSA)檢測試劑盒(高敏型)
貨 號：
HEA248Ge-48T
規(guī) 格：
48T
品 牌：
cloud-clone
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| 英文名稱： | High Sensitive ELISA Kit for Bovine Serum Albumin (BSA) |
| 基因別稱： | Bovine Albumin; Fraction V |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50μL standard or sample to each well. And then add 50μL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37oC; 7. Add 50μL Stop Solution. Read at 450 nm immediately. |
| 檢測范圍： | 246.9-20,000ng/mL. The standard curve concentrations used for the ELISA’s were 20,000ng/mL, 6,666.7ng/mL, 2,222.2ng/mL, 740.7ng/mL, 246.9ng/mL. |
| 適應(yīng)生物： | Pan-species (General，通用) |
| 樣本： | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 種類： | 競爭法 |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of BSA. No significant cross-reactivity or interference between BSA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between BSA and all the analogues, therefore, cross reaction may still exist. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BSA were tested 20
times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BSA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within
the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BSA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BSA and unlabeled BSA (Standards or samples) with the pre-coated antibody specific to BSA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BSA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BSA in the sample. |
| 產(chǎn)品應(yīng)用： | elisa |
| 應(yīng)用備注： | 1. We are only responsible for the kit itself, but not for the samples consumed during the assay. The user should
calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in
advance. 2. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. 3. Serum/plasma samples require about a 10,000 fold dilution. For example, to prepare a 1:10,000 dilution of sample, transfer 10μL of sample to 990μL PBS. This yields a 1:100 dilution. Next, dilute the 1:100 sample by transferring 10μL to 990μL PBS. You now have a 1:10,000 dilution of your sample. Mix thoroughly at each stage. Sample should be diluted by 0.01mol/L PBS(PH=7.0-7.2). 4. If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary. 5. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals. 6. Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products. 7. Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernates may not be detected by the kit. 8. Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results. |
| 線性： | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BSA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
| 回收率： | Matrices listed below were spiked with certain level of BSA and the recovery rates were calculated by comparing the measured value to the expected amount of BSA in samples. |
| 期貨： | 5-7個工作日 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 4度 |
| 有效期： | 6個月 |
| 限制： | 僅用于科研 |
